Chromatographic Separation of Aliphatic Monocarbonyl Compounds.

Chromatographic separation of glycated nucleotides.

A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an a...

Chromatographic Separation of the Diastereoisomers

Reduction of folic acid to the tetrahydro level introduces a second asymmetrical center into the molecule at carbon atom 6.1 The resulting tetrahydrofolate, and subsequent derivatives, prepared chemically consist of dl mixtures that are only 50% active in biological reactions. The need for fully reactive isomers of reduced folates led to the enzymatic synthesis of tetrahydrofolate from 7, %dihy...

High-performance Liquid Chromatographic Separation of the Enantiomers of Amino Compounds on Chiral Stationary Phases

Optimization of normal-phase chromatographic separation of compounds with primary, secondary and tertiary amino groups.

The retention behavior of primary, secondary and tertiary amines was studied using normal-phase-HPLC on silica, diol, and cyano stationary phases. Several classes of amines, including benzylamines, anilines, ephedrines, tryptamines, and azatryptamines were chromatographed using mixtures of hexane and ethoxynonafluorobutane with methylene chloride and methanol. Peak tailing, diminished selectivi...

New alkyl-phosphate bonded stationary phases for liquid chromatographic separation of biologically active compounds

A new type of bonded stationary phase for liquid chromatography, with the properties of immobilized artificial membranes, has been synthesized. Alkyl-phosphate adsorbents were obtained by modification of aminopropyl silica gel. The structures of the synthesized materials were confirmed by use of instrumental techniques--elemental analysis, infrared spectroscopy (FTIR), and (13)C and (29)Si CP/M...